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Image Search Results
Journal: PLoS ONE
Article Title: Identification and characterization of protein N -myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25
doi: 10.1371/journal.pone.0206355
Figure Lengend Snippet: Association of MIC19-FLAG stably expressed in COS-1 cells with endogenously expressed SAMM50 was studied by immunoprecipitation analysis. A. Expression of MIC19-FLAG and MIC19-G2A-FLAG in respective stable transformants. Upper panel; Western blotting analysis using an anti-FLAG antibody, Lower panel; Western blotting analysis using an anti-MIC19 antibody. B. Detection of protein N -myristoylation of MIC19-FLAG expressed in stable transformants of MIC19-FLAG. Upper panel; Western blotting analysis using an anti-FLAG antibody. Lower panel; Detection of protein N -myristoylation by metabolic labeling with myristic acid analog followed by detection with click chemistry. C. Western blotting analysis of MIC19-FLAG or MIC19-G2A-FLAG immunoprecipitated from stable transformants of MIC19-FLAG and MIC19-G2A-FLAG using an anti-MIC19 antibody. D. Analysis of the binding of MIC19-FLAG or MIC19-G2A-FLAG to endogenous SAMM50 by western blotting analysis of immunoprecipitated samples of stable transformants of MIC19-FLAG and MIC19-G2A-FLAG using an anti-SAMM50 antibody. The experiments were performed in triplicate. A typical pattern of western blotting is presented. E. Quantitative analysis of MIC19-FLAG or MIC19-G2A-FLAG bound to SAMM50 was performed using the results obtained in D. Data are expressed as mean ± SD for three independent experiments. * P < 0.01 vs. wild-type.
Article Snippet: The membrane was developed using ECL Prime or
Techniques: Stable Transfection, Immunoprecipitation, Expressing, Western Blot, Labeling, Binding Assay